| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DLC1 (204-463) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DLC1 |
| Gene ID: |
10395 |
| Uniprot ID: |
RHG07_HUMAN |
| Immunogen Region: |
204-463 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human DLC1 (NP_079043.3). |
| Immunogen Sequence: |
DAPKVNAVDTLNVKDIAPEK QLLNSAVIAQQRRKPDPPKD ENERSTCNVVQNEFLDTPCT NRGLPLLKTDFGSCLLQPPS CPNGMSAENGLEKSGFSQHQ NKSPPKVKAEDGMQCLQLKE TLATQEPTDNQVRLRKRKEI REDRDRARLDSMVLLIMKLD QLDQDIENALSTSSSPSGTP TNLRRHVPDLESGSESGADT ISVNQTRVNLSSDTESTDLP SSTPVANSGTKPKTTAIQG |
| Tissue Specificity | Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus. |
| Function | Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Required for growth factor-induced epithelial cell migration.in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85. The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation. |
| Protein Name | Rho Gtpase-Activating Protein 7Deleted In Liver Cancer 1 ProteinDlc-1Hp ProteinRho-Type Gtpase-Activating Protein 7Start Domain-Containing Protein 12Stard12Star-Related Lipid Transfer Protein 12 |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406 |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionMembranePeripheral Membrane ProteinColocalizes With Ef1a1 At Actin-Rich Regions In The Cell Periphery |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 7 antibodyAnti-Deleted In Liver Cancer 1 Protein antibodyAnti-Dlc-1 antibodyAnti-Hp Protein antibodyAnti-Rho-Type Gtpase-Activating Protein 7 antibodyAnti-Start Domain-Containing Protein 12 antibodyAnti-Stard12 antibodyAnti-Star-Related Lipid Transfer Protein 12 antibodyAnti-DLC1 antibodyAnti-ARHGAP7 antibodyAnti-KIAA1723 antibodyAnti-STARD12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/93811/375003/STJ114724_1__02265.1713141659.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
