| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Di-Methyl-DNMT3A-K44 is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Dnmt3a |
| Gene ID: |
13435 |
| Uniprot ID: |
DNM3A_MOUSE |
| Immunogen: |
A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1). |
| Immunogen Sequence: |
ARKVG |
| Tissue Specificity | Isoform 1 is expressed ubiquitously at low levels. Expression of isoform 2 is restricted to tissues containing cells which are undergoing active de novo methylation, including spleen, testis and thymus. |
| Post Translational Modifications | Auto-methylated at Cys-706: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-706 in absence of DNA. |
| Protein Name | DnaCytosine-5-Methyltransferase 3aDnmt3aCysteine Methyltransferase Dnmt3aDna Methyltransferase MmuiiiaDna Mtase MmuiiiaM.mmuiiia |
| Database Links | Reactome: R-MMU-212300Reactome: -MMU-3214858 |
| Cellular Localisation | NucleusChromosomeCytoplasmAccumulates In The Major Satellite Repeats At Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3a antibodyAnti-Dnmt3a antibodyAnti-Cysteine Methyltransferase Dnmt3a antibodyAnti-Dna Methyltransferase Mmuiiia antibodyAnti-Dna Mtase Mmuiiia antibodyAnti-M.mmuiiia antibodyAnti-Dnmt3a antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
