Anti-DENND1B antibody (325-375 aa) (STJ194999)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human DEN1B at the amino acid range 325-375
General Information
| Short Description | Rabbit polyclonal anti-DENN domain-containing protein 1B (325-375 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DENND1B |
| Gene ID | 163486 |
| Uniprot ID | DEN1B_HUMAN |
| Immunogen | Synthesized peptide derived from the human DEN1B at the amino acid range 325-375 |
| Immunogen Region | 325-375 aa |
| Specificity | This antibody detects endogenous levels of DEN1B at Human/Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and/or threonine, possibly regulating the guanine nucleotide exchange factor (GEF) activity. |
| Function | Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells. |
| Protein Name | Denn Domain-Containing Protein 1bConnecdenn 2Protein Fam31b |
| Database Links | Reactome: R-HSA-8876198 |
| Cellular Localisation | CytoplasmCytosolCytoplasmic VesicleClathrin-Coated Vesicle |
| Alternative Antibody Names | Anti-Denn Domain-Containing Protein 1b antibodyAnti-Connecdenn 2 antibodyAnti-Protein Fam31b antibodyAnti-DENND1B antibodyAnti-C1orf218 antibodyAnti-FAM31B antibody |
Information sourced from Uniprot.org