DDDDK-Tag Antibodies
Detection of DDDDK (FLAG) tagged fusion proteins, with a built-in enterokinase cleavage siteThe DDDDK-Tag is the enterokinase recognition sequence within the eight-residue FLAG epitope (DYKDDDDK), first described by Hopp and colleagues in 1988 as a short, hydrophilic purification handle that also carries a site for complete removal of the tag by enterokinase cleavage after purification.1
Although later work explored optimising the sequence by phage display or ELISA screening, the original DYKDDDDK sequence is still used for almost all FLAG-tagged constructs.2 Our range covers both the polyclonal and monoclonal formats for this tag, across Western Blot, immunohistochemistry, immunofluorescence and immunoprecipitation.
Anti-DDDDK-Tag Antibodies
Polyclonal and monoclonal antibodies against the DDDDK (FLAG) tag, validated for Western Blot, IHC, IF and immunoprecipitation.
Looking for a Different Tag?
We supply antibodies against DDDDK (FLAG) Tag, His-Tag, HA-Tag, Avi-Tag, Myc-Tag, GST-Tag, V5-Tag, T7-Tag, Strep-Tag, S-Tag, GFP-Tag and mCherry-Tag fusion proteins.
References
1. Klotz A, et al. Taking down the FLAG! How Insect Cell Expression Challenges an Established Tag-System. PLoS ONE. Link
2. Zhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093. Link
For Research Use Only. Not for use in diagnostic or therapeutic procedures.