Anti-DDAH2 antibody (1-285) (STJ28540)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DDAH2 (1-285) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DDAH2 |
| Gene ID | 23564 |
| Uniprot ID | DDAH2_HUMAN |
| Immunogen Region | 1-285 |
| Immunogen Sequence | MGTPGEGLGRCSHALIRGVP ESLASGEGAGAGLPALDLAK AQREHGVLGGKLRQRLGLQL LELPPEESLPLGPLLGDTAV IQGDTALITRPWSPARRPEV DGVRKALQDLGLRIVEIGDE NATLDGTDVLFTGREFFVGL SKWTNHRGAEIVADTFRDFA VSTVPVSGPSHLRGLCGMGG PRTVVAGSSDAAQKAVRAMA VLTDHPYASLTLPDDAAADC LFLRPGLPGVPPFLLHRGG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1). |
Additional Info
| Tissue Specificity | Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. |
| Post Translational Modifications | Phosphorylated by TBK1. Phosphorylation inhibits the translocation into the mitochondrion upon Sendai viral infection. |
| Function | Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS. In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase. Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein. Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS. |
| Protein Name | Putative Hydrolase Ddah2DdahiiInactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2Ddah-2Inactive Dimethylarginine Dimethylaminohydrolase 2Protein G6aS-Phase Protein |
| Database Links | |
| Cellular Localisation | CytoplasmMitochondrionTranslocates From Cytosol To Mitochondrion Upon Il1b Stimulation In Chondrocytes(Microbial Infection) Translocates To The Mitochondrion Upon Sendai Viral Infection |
| Alternative Antibody Names | Anti-Putative Hydrolase Ddah2 antibodyAnti-Ddahii antibodyAnti-Inactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2 antibodyAnti-Ddah-2 antibodyAnti-Inactive Dimethylarginine Dimethylaminohydrolase 2 antibodyAnti-Protein G6a antibodyAnti-S-Phase Protein antibodyAnti-DDAH2 antibodyAnti-DDAH antibodyAnti-G6A antibodyAnti-NG30 antibody |
Information sourced from Uniprot.org