| Tissue Specificity | Expressed in brain and testis. Not detected in heart (at protein level). |
| Post Translational Modifications | Phosphorylated at ser-249 in a MTOR-dependent manner. |
| Function | Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap.m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts. |
| Protein Name | M7gpppn-Mrna HydrolaseNucleoside Diphosphate-Linked Moiety X Motif 20Nudix Motif 20Mrna-Decapping Enzyme 2Hdpc |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-430039Reactome: R-HSA-450385Reactome: R-HSA-450513Reactome: R-HSA-450604 |
| Cellular Localisation | CytoplasmP-BodyNucleusPredominantly CytoplasmicIn Processing Bodies (Pb)A Minor Amount Is Nuclear |
| Alternative Antibody Names | Anti-M7gpppn-Mrna Hydrolase antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 20 antibodyAnti-Nudix Motif 20 antibodyAnti-Mrna-Decapping Enzyme 2 antibodyAnti-Hdpc antibodyAnti-DCP2 antibodyAnti-NUDT20 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89138/357587/STJ11100068_1__03762.1713121722.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
