Anti-DCP2 antibody (1-240) (STJ110581)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DCP2 (1-240) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DCP2 |
| Gene ID | 167227 |
| Uniprot ID | DCP2_HUMAN |
| Immunogen Region | 1-240 |
| Immunogen Sequence | METKRVEIPGSVLDDLCSRF ILHIPSEERDNAIRVCFQIE LAHWFYLDFYMQNTPGLPQC GIRDFAKAVFSHCPFLLPQG EDVEKVLDEWKEYKMGVPTY GAIILDETLENVLLVQGYLA KSGWGFPKGKVNKEEAPHDC AAREVFEETGFDIKDYICKD DYIELRINDQLARLYIIPGI PKDTKFNPKTRREIRNIEWF SIEKLPCHRNDMTPKSKLGL APNKFFMAIPFIRPLRDWL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1). |
Additional Info
| Tissue Specificity | Expressed in brain and testis. Not detected in heart (at protein level). |
| Post Translational Modifications | Phosphorylated at ser-249 in a MTOR-dependent manner. |
| Function | Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap.m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts. |
| Protein Name | M7gpppn-Mrna HydrolaseNucleoside Diphosphate-Linked Moiety X Motif 20Nudix Motif 20Mrna-Decapping Enzyme 2Hdpc |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-430039Reactome: R-HSA-450385Reactome: R-HSA-450513Reactome: R-HSA-450604 |
| Cellular Localisation | CytoplasmP-BodyNucleusPredominantly CytoplasmicIn Processing Bodies (Pb)A Minor Amount Is Nuclear |
| Alternative Antibody Names | Anti-M7gpppn-Mrna Hydrolase antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 20 antibodyAnti-Nudix Motif 20 antibodyAnti-Mrna-Decapping Enzyme 2 antibodyAnti-Hdpc antibodyAnti-DCP2 antibodyAnti-NUDT20 antibody |
Information sourced from Uniprot.org