• Immunofluorescence analysis of HeLa cells using DCK antibody (STJ23344). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using DCK antibody (STJ23344) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using DCK Rabbit polyclonal antibody (STJ23344) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DCK antibody (1-260) (STJ23344)

SKU:
STJ23344

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCK (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCK
Gene ID: 1633
Uniprot ID: DCK_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1).
Immunogen Sequence: MATPPKRSCPSFSASSEGTR IKKISIEGNIAAGKSTFVNI LKQLCEDWEVVPEPVARWCN VQSTQDEFEELTMSQKNGGN VLQMMYEKPERWSFTFQTYA CLSRIRAQLASLNGKLKDAE KPVLFFERSVYSDRYIFASN LYESECMNETEWTIYQDWHD WMNNQFGQSLELDGIIYLQA TPETCLHRIYLRGRNEEQGI PLEYLEKLHYKHESWLLHRT LKTNFDYLQEVPILTLDVN
Post Translational Modifications Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.
Function Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine. Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Protein Name Deoxycytidine Kinase
Dck
Deoxyadenosine Kinase
Deoxyguanosine Kinase
Database Links Reactome: R-HSA-73614
Reactome: R-HSA-74217
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Deoxycytidine Kinase antibody
Anti-Dck antibody
Anti-Deoxyadenosine Kinase antibody
Anti-Deoxyguanosine Kinase antibody
Anti-DCK antibody

Information sourced from Uniprot.org

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