![Western blot analysis of various lysates using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186555/408225/STJ11107359_1__82817.1744894610.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and DCK knockdown (KD) 293F cells using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186555/408226/STJ11107359_2__10175.1744894610.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DCK knockdown (KD) 293F cells using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186555/408225/STJ11107359_1__82817.1744894610.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] DCK Rabbit mAb (STJ11107359) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1s.")
Anti-DCK antibody (1-260 aa) [SRM5446] (STJ11107359)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal DCK (1-260 aa) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5446 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:4000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DCK |
| Gene ID | 1633 |
| Uniprot ID | DCK_HUMAN |
| Immunogen Region | 1-260 aa |
| Immunogen Sequence | MATPPKRSCPSFSASSEGTR IKKISIEGNIAAGKSTFVNI LKQLCEDWEVVPEPVARWCN VQSTQDEFEELTMSQKNGGN VLQMMYEKPERWSFTFQTYA CLSRIRAQLASLNGKLKDAE KPVLFFERSVYSDRYIFASN LYESECMNETEWTIYQDWHD WMNNQFGQSLELDGIIYLQA TPETCLHRIYLRGRNEEQGI PLEYLEKLHYKHESWLLHRT LKTNFDYLQEVPILTLDVN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1). |
Additional Info
| Post Translational Modifications | Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1. |
| Function | Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine. Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. |
| Protein Name | Deoxycytidine KinaseDckDeoxyadenosine KinaseDeoxyguanosine Kinase |
| Database Links | Reactome: R-HSA-73614Reactome: R-HSA-74217 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Deoxycytidine Kinase antibodyAnti-Dck antibodyAnti-Deoxyadenosine Kinase antibodyAnti-Deoxyguanosine Kinase antibodyAnti-DCK antibody |
Information sourced from Uniprot.org