Anti-DCAF12 antibody (1-85) (STJ118937)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DCAF12 (1-85) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DCAF12 |
| Gene ID | 25853 |
| Uniprot ID | DCA12_HUMAN |
| Immunogen Region | 1-85 |
| Immunogen Sequence | MARKVVSRKRKAPASPGAGS DAQGPQFGWDHSLHKRKRLP PVKRSLVYYLKNREVRLQNE TSYSRVLHGYAAQQLPSLLK EREFH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human DCAF12 (NP_056212.1). |
Additional Info
| Tissue Specificity | Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis. |
| Function | Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation. Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy. Also directly recognizes the C-terminal glutamate-leucine (Glu-Leu) degron as an alternative degron in proteins such as MOV10, leading to their ubiquitination and degradation. Controls the protein level of MOV10 during spermatogenesis and in T cells, especially after their activation. |
| Protein Name | Ddb1- And Cul4-Associated Factor 12Centrosome-Related Protein Tcc52Testis Cancer Centrosome-Related ProteinWd Repeat-Containing Protein 40a |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus |
| Alternative Antibody Names | Anti-Ddb1- And Cul4-Associated Factor 12 antibodyAnti-Centrosome-Related Protein Tcc52 antibodyAnti-Testis Cancer Centrosome-Related Protein antibodyAnti-Wd Repeat-Containing Protein 40a antibodyAnti-DCAF12 antibodyAnti-KIAA1892 antibodyAnti-TCC52 antibodyAnti-WDR40A antibody |
Information sourced from Uniprot.org