| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DCAF12 (1-85) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DCAF12 |
| Gene ID: |
25853 |
| Uniprot ID: |
DCA12_HUMAN |
| Immunogen Region: |
1-85 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human DCAF12 (NP_056212.1). |
| Immunogen Sequence: |
MARKVVSRKRKAPASPGAGS DAQGPQFGWDHSLHKRKRLP PVKRSLVYYLKNREVRLQNE TSYSRVLHGYAAQQLPSLLK EREFH |
| Tissue Specificity | Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis. |
| Function | Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation. Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy. Also directly recognizes the C-terminal glutamate-leucine (Glu-Leu) degron as an alternative degron in proteins such as MOV10, leading to their ubiquitination and degradation. Controls the protein level of MOV10 during spermatogenesis and in T cells, especially after their activation. |
| Protein Name | Ddb1- And Cul4-Associated Factor 12Centrosome-Related Protein Tcc52Testis Cancer Centrosome-Related ProteinWd Repeat-Containing Protein 40a |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus |
| Alternative Antibody Names | Anti-Ddb1- And Cul4-Associated Factor 12 antibodyAnti-Centrosome-Related Protein Tcc52 antibodyAnti-Testis Cancer Centrosome-Related Protein antibodyAnti-Wd Repeat-Containing Protein 40a antibodyAnti-DCAF12 antibodyAnti-KIAA1892 antibodyAnti-TCC52 antibodyAnti-WDR40A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
