Anti-DBT antibody (11-247) (STJ11103287)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DBT (11-247) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DBT |
| Gene ID | 1629 |
| Uniprot ID | ODB2_HUMAN |
| Immunogen Region | 11-247 |
| Immunogen Sequence | SRNAGKLICVRYFQTCGNVH VLKPNYVCFFGYPSFKYSHP HHFLKTTAALRGQVVQFKLS DIGEGIREVTVKEWYVKEGD TVSQFDSICEVQSDKASVTI TSRYDGVIKKLYYNLDDIAY VGKPLVDIETEALKDSEEDV VETPAVSHDEHTHQEIKGRK TLATPAVRRLAMENNIKLSE VVGSGKDGRILKEDILNYLE KQTGAILPPSPKVEIMPPPP KPKDMTVPILVSKPPVF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3). |
Additional Info
| Function | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. |
| Protein Name | Lipoamide Acyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex - Mitochondrial52 Kda Mitochondrial Autoantigen Of Primary Biliary CirrhosisBranched Chain 2-Oxo-Acid Dehydrogenase Complex Component E2Bcoadc-E2Branched-Chain Alpha-Keto Acid Dehydrogenase Complex Component E2Bckad-E2Bckade2Bckdh-E2Dihydrolipoamide Acetyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase ComplexDihydrolipoamide Branched Chain TransacylaseDihydrolipoyllysine-Residue2-Methylpropanoyltransferase |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9013407Reactome: R-HSA-9837999Reactome: R-HSA-9857492Reactome: R-HSA-9859138Reactome: R-HSA-9865113Reactome: R-HSA-9865125Reactome: R-HSA-9907570Reactome: R-HSA-9912481Reactome: R-HSA-9912529 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Lipoamide Acyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex - Mitochondrial antibodyAnti-52 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis antibodyAnti-Branched Chain 2-Oxo-Acid Dehydrogenase Complex Component E2 antibodyAnti-Bcoadc-E2 antibodyAnti-Branched-Chain Alpha-Keto Acid Dehydrogenase Complex Component E2 antibodyAnti-Bckad-E2 antibodyAnti-Bckade2 antibodyAnti-Bckdh-E2 antibodyAnti-Dihydrolipoamide Acetyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex antibodyAnti-Dihydrolipoamide Branched Chain Transacylase antibodyAnti-Dihydrolipoyllysine-Residue antibodyAnti-2-Methylpropanoyltransferase antibodyAnti-DBT antibodyAnti-BCATE2 antibodyAnti-BCKDHE2 antibody |
Information sourced from Uniprot.org