• Western blot analysis of extracts of various cell lines, using DBT antibody (STJ11103287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DBT rabbit polyclonal antibody (STJ11103287) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-431 cells using DBT rabbit polyclonal antibody (STJ11103287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DBT rabbit polyclonal antibody (STJ11103287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DBT antibody (11-247) (STJ11103287)

SKU:
STJ11103287

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DBT (11-247) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DBT
Gene ID: 1629
Uniprot ID: ODB2_HUMAN
Immunogen Region: 11-247
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 11-247 of human DBT (NP_001909.3).
Immunogen Sequence: SRNAGKLICVRYFQTCGNVH VLKPNYVCFFGYPSFKYSHP HHFLKTTAALRGQVVQFKLS DIGEGIREVTVKEWYVKEGD TVSQFDSICEVQSDKASVTI TSRYDGVIKKLYYNLDDIAY VGKPLVDIETEALKDSEEDV VETPAVSHDEHTHQEIKGRK TLATPAVRRLAMENNIKLSE VVGSGKDGRILKEDILNYLE KQTGAILPPSPKVEIMPPPP KPKDMTVPILVSKPPVF
Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
Protein Name Lipoamide Acyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex - Mitochondrial
52 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis
Branched Chain 2-Oxo-Acid Dehydrogenase Complex Component E2
Bcoadc-E2
Branched-Chain Alpha-Keto Acid Dehydrogenase Complex Component E2
Bckad-E2
Bckade2
Dihydrolipoamide Acetyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex
Dihydrolipoamide Branched Chain Transacylase
Dihydrolipoyllysine-Residue
2-Methylpropanoyltransferase
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-70895
Reactome: R-HSA-9013407
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Lipoamide Acyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex - Mitochondrial antibody
Anti-52 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis antibody
Anti-Branched Chain 2-Oxo-Acid Dehydrogenase Complex Component E2 antibody
Anti-Bcoadc-E2 antibody
Anti-Branched-Chain Alpha-Keto Acid Dehydrogenase Complex Component E2 antibody
Anti-Bckad-E2 antibody
Anti-Bckade2 antibody
Anti-Dihydrolipoamide Acetyltransferase Component Of Branched-Chain Alpha-Keto Acid Dehydrogenase Complex antibody
Anti-Dihydrolipoamide Branched Chain Transacylase antibody
Anti-Dihydrolipoyllysine-Residue antibody
Anti-2-Methylpropanoyltransferase antibody
Anti-DBT antibody
Anti-BCATE2 antibody

Information sourced from Uniprot.org

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