• Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from NIH/3T3 cells, treated with H2O2 100uM 30', COS7 treated with PMA 125ng/ml 30', using DBF4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Dbf4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DBF4 antibody (10-59 aa) (STJ92660)

SKU:
STJ92660

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein DBF4 homolog A (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DBF4
Gene ID: 10926
Uniprot ID: DBF4A_HUMAN
Immunogen Region: 10-59 aa
Specificity: Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DBF4 at the amino acid range 10-59
Post Translational Modifications Phosphorylation increases its interaction with PSIP1.
Function Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Protein Name Protein Dbf4 Homolog A
Activator Of S Phase Kinase
Chiffon Homolog A
Dbf4-Type Zinc Finger-Containing Protein 1
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Dbf4 Homolog A antibody
Anti-Activator Of S Phase Kinase antibody
Anti-Chiffon Homolog A antibody
Anti-Dbf4-Type Zinc Finger-Containing Protein 1 antibody
Anti-DBF4 antibody
Anti-ASK antibody
Anti-DBF4A antibody
Anti-ZDBF1 antibody

Information sourced from Uniprot.org

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