• Western blot analysis of extracts of various cell lines, using DBF4 Rabbit monoclonal antibody (STJ11103121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DBF4 antibody (1-100) [S1MR] (STJ11103121)

SKU:
STJ11103121

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DBF4 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DBF4
Gene ID: 10926
Uniprot ID: DBF4A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DBF4 (Q9UBU7).
Immunogen Sequence: MNSGAMRIHSKGHFQGGIQV KNEKNRPSLKSLKTDNRPEK SKCKPLWGKVFYLDLPSVTI SEKLQKDIKDLGGRVEEFLS KDISYLISNKKEAKFAQTLG
Tissue Specificity Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Post Translational Modifications Phosphorylation increases its interaction with PSIP1.
Function Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Protein Name Protein Dbf4 Homolog A
Activator Of S Phase Kinase
Chiffon Homolog A
Dbf4-Type Zinc Finger-Containing Protein 1
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Dbf4 Homolog A antibody
Anti-Activator Of S Phase Kinase antibody
Anti-Chiffon Homolog A antibody
Anti-Dbf4-Type Zinc Finger-Containing Protein 1 antibody
Anti-DBF4 antibody
Anti-ASK antibody
Anti-DBF4A antibody
Anti-ZDBF1 antibody

Information sourced from Uniprot.org

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