Anti-CYP51A1 antibody (245-420) (STJ115446)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYP51A1 (245-420) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP51A1 |
| Gene ID | 1595 |
| Uniprot ID | CP51A_HUMAN |
| Immunogen Region | 245-420 |
| Immunogen Sequence | WLLPGWLPLPSFRRRDRAHR EIKDIFYKAIQKRRQSQEKI DDILQTLLDATYKDGRPLTD DEVAGMLIGLLLAGQHTSST TSAWMGFFLARDKTLQKKCY LEQKTVCGENLPPLTYDQLK DLNLLDRCIKETLRLRPPIM IMMRMARTPQTVAGYTIPPG HQVCVSPTVNQRLKDS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 245-420 of CYP51A1 (NP_000777.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung. |
| Post Translational Modifications | Ubiquitinated by MARCHF6, leading to proteasomal degradation. |
| Function | Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis. Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL. |
| Protein Name | Lanosterol 14-Alpha DemethylaseLdmCypliCytochrome P450 51a1Cyp51a1Cytochrome P450-14dmCytochrome P45014dmCytochrome P450liSterol 14-Alpha Demethylase |
| Database Links | Reactome: R-HSA-191273Reactome: R-HSA-211976Reactome: R-HSA-2426168Reactome: R-HSA-9619665 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Lanosterol 14-Alpha Demethylase antibodyAnti-Ldm antibodyAnti-Cypli antibodyAnti-Cytochrome P450 51a1 antibodyAnti-Cyp51a1 antibodyAnti-Cytochrome P450-14dm antibodyAnti-Cytochrome P45014dm antibodyAnti-Cytochrome P450li antibodyAnti-Sterol 14-Alpha Demethylase antibodyAnti-CYP51A1 antibodyAnti-CYP51 antibody |
Information sourced from Uniprot.org