| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP51A1 |
| Gene ID: | 1595 |
| Uniprot ID: | CP51A_HUMAN |
| Immunogen Region: | 245-420 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-420 of CYP51A1 (NP_000777.1). |
| Immunogen Sequence: | WLLPGWLPLPSFRRRDRAHR EIKDIFYKAIQKRRQSQEKI DDILQTLLDATYKDGRPLTD DEVAGMLIGLLLAGQHTSST TSAWMGFFLARDKTLQKKCY LEQKTVCGENLPPLTYDQLK DLNLLDRCIKETLRLRPPIM IMMRMARTPQTVAGYTIPPG HQVCVSPTVNQRLKDS |
| Tissue Specificity | Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung. |
| Function | Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis. Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL. |
| Protein Name | Lanosterol 14-Alpha Demethylase Ldm Cypli Cytochrome P450 51a1 Cyp51a1 Cytochrome P450-14dm Cytochrome P45014dm Cytochrome P450li Sterol 14-Alpha Demethylase |
| Database Links | Reactome: R-HSA-191273 Reactome: R-HSA-211976 Reactome: R-HSA-2426168 Reactome: R-HSA-9619665 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Microsome Membrane |
| Alternative Antibody Names | Anti-Lanosterol 14-Alpha Demethylase antibody Anti-Ldm antibody Anti-Cypli antibody Anti-Cytochrome P450 51a1 antibody Anti-Cyp51a1 antibody Anti-Cytochrome P450-14dm antibody Anti-Cytochrome P45014dm antibody Anti-Cytochrome P450li antibody Anti-Sterol 14-Alpha Demethylase antibody Anti-CYP51A1 antibody Anti-CYP51 antibody |
Information sourced from Uniprot.org