Western blot analysis of lysates from Rat testis, using Cyclin B2 Rabbit mAb (STJ11103618) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Cyclin B2 antibody (1-100) [S8MR] (STJ11103618)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103618
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General Information

Short DescriptionRabbit monoclonal Cyclin B2 (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCCNB2
Gene ID9133
Uniprot IDCCNB2_HUMAN
Immunogen Region1-100
Immunogen SequenceMALLRRPTVSSDLENIDTGV NSKVKSHVTIRRTVLEEIGN RVTTRAAQVAKKAQNTKVPV QPTKTTNVNKQLKPTASVKP VQMEKLAPKGPSPTPEDVSM
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cyclin B2 (O95067).

Additional Info

Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Protein Name G2/Mitotic-Specific Cyclin-B2
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-162658
Reactome: R-HSA-2500257
Reactome: R-HSA-2514853
Reactome: R-HSA-2565942
Reactome: R-HSA-2980767
Reactome: R-HSA-2995383
Reactome: R-HSA-3301854
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-8852276
Cellular Localisation
Alternative Antibody Names Anti-G2/Mitotic-Specific Cyclin-B2 antibody
Anti-CCNB2 antibody

Information sourced from Uniprot.org

Citations

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