• Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (STJ11103618) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Confocal imaging of NIH/3T3 cells using Cyclin B2 Rabbit monoclonal antibody (STJ11103618, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin B2 rabbit monoclonal antibody (STJ11103618) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Cyclin B2 in paraffin-embedded human liver cancer using Cyclin B2 Rabbit monoclonal antibody (STJ11103618) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Cyclin B2 rabbit monoclonal antibody (STJ11103618) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat testis, using Cyclin B2 Rabbit monoclonal antibody (STJ11103618) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Cyclin B2 antibody (1-100) [S8MR] (STJ11103618)

SKU:
STJ11103618

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cyclin B2 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNB2
Gene ID: 9133
Uniprot ID: CCNB2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cyclin B2 (O95067).
Immunogen Sequence: MALLRRPTVSSDLENIDTGV NSKVKSHVTIRRTVLEEIGN RVTTRAAQVAKKAQNTKVPV QPTKTTNVNKQLKPTASVKP VQMEKLAPKGPSPTPEDVSM
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Protein Name G2/Mitotic-Specific Cyclin-B2
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-162658
Reactome: R-HSA-2500257
Reactome: R-HSA-2514853
Reactome: R-HSA-2565942
Reactome: R-HSA-2980767
Reactome: R-HSA-2995383
Reactome: R-HSA-3301854
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-8852276
Alternative Antibody Names Anti-G2/Mitotic-Specific Cyclin-B2 antibody
Anti-CCNB2 antibody

Information sourced from Uniprot.org

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