Western blot analysis of various lysates using CXCR5 Rabbit mAb (STJ11102401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CXCR5 antibody (273-372) [S1MR] (STJ11102401)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102401
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General Information

Short DescriptionRabbit monoclonal CXCR5 (273-372) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS1MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
IF/ICC:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCXCR5
Gene ID643
Uniprot IDCXCR5_HUMAN
Immunogen Region273-372
Immunogen SequenceSPYHIVIFLDTLARLKAVDN TCKLNGSLPVAITMCEFLGL AHCCLNPMLYTFAGVKFRSD LSRLLTKLGCTGPASLCQLF PSWRRSSLSESENATSLTTF
SpecificityA synthetic peptide corresponding to a sequence within amino acids 273-372 of human CXCR5 (P32302).

Additional Info

Tissue Specificity Expression in mature B-cells and Burkitt lymphoma cells.
Function Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Protein Name C-X-C Chemokine Receptor Type 5
Cxc-R5
Cxcr-5
Burkitt Lymphoma Receptor 1
Monocyte-Derived Receptor 15
Mdr-15
Cd Antigen Cd185
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-C-X-C Chemokine Receptor Type 5 antibody
Anti-Cxc-R5 antibody
Anti-Cxcr-5 antibody
Anti-Burkitt Lymphoma Receptor 1 antibody
Anti-Monocyte-Derived Receptor 15 antibody
Anti-Mdr-15 antibody
Anti-Cd Antigen Cd185 antibody
Anti-CXCR5 antibody
Anti-BLR1 antibody
Anti-MDR15 antibody

Information sourced from Uniprot.org

Citations

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