• Immunofluorescence analysis of HeLa cells using CTSS Rabbit polyclonal antibody (STJ23285) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using CTSS Rabbit polyclonal antibody (STJ23285) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CTSS Rabbit polyclonal antibody (STJ23285) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CTSS antibody (115-331) (STJ23285)

SKU:
STJ23285

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSS (115-331) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSS
Gene ID: 1520
Uniprot ID: CATS_HUMAN
Immunogen Region: 115-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-331 of human CTSS (NP_004070.3).
Immunogen Sequence: LPDSVDWREKGCVTEVKYQG SCGACWAFSAVGALEAQLKL KTGKLVSLSAQNLVDCSTEK YGNKGCNGGFMTTAFQYIID NKGIDSDASYPYKAMDQKCQ YDSKYRAATCSKYTELPYGR EDVLKEAVANKGPVSVGVDA RHPSFFLYRSGVYYEPSCTQ NVNHGVLVVGYGDLNGKEYW LVKNSWGHNFGEEGYIRMAR NKGNHCGIASFPSYPEI
Function Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Protein Name Cathepsin S
Database Links Reactome: R-HSA-1236977
Reactome: R-HSA-1474228
Reactome: R-HSA-1679131
Reactome: R-HSA-2022090
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Secreted
Cytoplasmic Vesicle
Phagosome
Alternative Antibody Names Anti-Cathepsin S antibody
Anti-CTSS antibody

Information sourced from Uniprot.org

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