• Immunofluorescence analysis of THP-1 cells using Cathepsin G Rabbit polyclonal antibody (STJ27603) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cathepsin G in paraffin-embedded rat spleen using Cathepsin G Rabbit polyclonal antibody (STJ27603) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cathepsin G in paraffin-embedded mouse spleen using Cathepsin G Rabbit polyclonal antibody (STJ27603) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cathepsin G in paraffin-embedded human tonsil using Cathepsin G Rabbit polyclonal antibody (STJ27603) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cathepsin G Rabbit polyclonal antibody (STJ27603) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from Raji cells, using Cathepsin G Rabbit polyclonal antibody (STJ27603) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CTSG antibody (21-255) (STJ27603)

SKU:
STJ27603

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSG (21-255) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSG
Gene ID: 1511
Uniprot ID: CATG_HUMAN
Immunogen Region: 21-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-255 of human Cathepsin G (NP_001902.1).
Immunogen Sequence: IIGGRESRPHSRPYMAYLQI QSPAGQSRCGGFLVREDFVL TAAHCWGSNINVTLGAHNIQ RRENTQQHITARRAIRHPQY NQRTIQNDIMLLQLSRRVRR NRNVNPVALPRAQEGLRPGT LCTVAGWGRVSMRRGTDTLR EVQLRVQRDRQCLRIFGSYD PRRQICVGDRRERKAAFKGD SGGPLLCNNVAHGIVSYGKS SGVPPEVFTRVSSFLPWIRT TMRSFKLLDQMETPL
Tissue Specificity Expressed in neutrophils (at protein level). Expressed in B cells.
Post Translational Modifications Two C-terminal truncation variants have been identified, one which ends at Arg-243 and one which ends at Ser-244.
Function Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor. Binds reversibly to and stimulates B cells and CD4(+) and CD8(+) T cells. Also binds reversibly to natural killer (NK) cells and enhances NK cell cytotoxicity through its protease activity. Cleaves complement C3. Cleaves vimentin. Cleaves thrombin receptor F2R/PAR1 and acts as either an agonist or an inhibitor, depending on the F2R cleavage site. Cleavage of F2R at '41-Arg-|-Ser-42' results in receptor activation while cleavage at '55-Phe-|-Trp-56' results in inhibition of receptor activation. Cleaves the synovial mucin-type protein PRG4/lubricin. Cleaves and activates IL36G which promotes expression of chemokines CXCL1 and CXLC8 in keratinocytes. Cleaves IL33 into mature forms which have greater activity than the unprocessed form. Cleaves coagulation factor F8 to produce a partially activated form. Also cleaves and activates coagulation factor F10. Cleaves leukocyte cell surface protein SPN/CD43 to releases its extracellular domain and trigger its intramembrane proteolysis by gamma-secretase, releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. Cleaves CCL5/RANTES to produce RANTES(4-68) lacking the N-terminal three amino acids which exhibits reduced chemotactic and antiviral activities. During apoptosis, cleaves SMARCA2/BRM to produce a 160 kDa cleavage product which localizes to the cytosol. Cleaves myelin basic protein MBP in B cell lysosomes at '224-Phe-|-Lys-225' and '248-Phe-|-Ser-249', degrading the major immunogenic MBP epitope and preventing the activation of MBP-specific autoreactive T cells. Cleaves annexin ANXA1 and antimicrobial peptide CAMP to produce peptides which act on neutrophil N-formyl peptide receptors to enhance the release of CXCL2. Acts as a ligand for the N-formyl peptide receptor FPR1, enhancing phagocyte chemotaxis. Has antibacterial activity against the Gram-negative bacteria N.gonorrhoeae and P.aeruginosa. Likely to act against N.gonorrhoeae by interacting with N.gonorrhoeae penA/PBP2. Exhibits potent antimicrobial activity against the Gram-positive bacterium L.monocytogenes. Has antibacterial activity against the Gram-positive bacterium S.aureus and degrades S.aureus biofilms, allowing polymorphonuclear leukocytes to penetrate the biofilm and phagocytose bacteria. Has antibacterial activity against M.tuberculosis. Mediates CASP4 activation induced by the Td92 surface protein of the periodontal pathogen T.denticola, causing production and secretion of IL1A and leading to pyroptosis of gingival fibroblasts.
Protein Name Cathepsin G
Cg Cleaved Into - Cathepsin G - C-Terminal Truncated Form
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022377
Reactome: R-HSA-381426
Reactome: R-HSA-448706
Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Reactome: R-HSA-9635465
Reactome: R-HSA-9660826
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Granule
Secreted
Cytoplasm
Cytosol
Lysosome
Nucleus
Secreted By Activated Neutrophils
Detected In Synovial Fluid
Localizes To Lysosomes In B Cells Where It Is Not Endogenously Synthesized But Is Internalized From The Cell Membrane
Localizes To The Nucleus During Apoptosis
Alternative Antibody Names Anti-Cathepsin G antibody
Anti-Cg Cleaved Into - Cathepsin G - C-Terminal Truncated Form antibody
Anti-CTSG antibody

Information sourced from Uniprot.org

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