• Immunofluorescence analysis of U2OS cells using CTNNBL1 Rabbit polyclonal antibody (STJ28655). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CTNNBL1 Rabbit polyclonal antibody (STJ28655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CTNNBL1 antibody (264-563) (STJ28655)

SKU:
STJ28655

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTNNBL1 (264-563) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTNNBL1
Gene ID: 56259
Uniprot ID: CTBL1_HUMAN
Immunogen Region: 264-563
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 264-563 of human CTNNBL1 (NP_110517.2).
Immunogen Sequence: EVLAILLQDNDENRELLGEL DGIDVLLQQLSVFKRHNPST AEEQEMMENLFDSLCSCLML SSNRERFLKGEGLQLMNLML REKKISRSSALKVLDHAMIG PEGTDNCHKFVDILGLRTIF PLFMKSPRKIKKVGTTEKEH EEHVCSILASLLRNLRGQQR TRLLNKFTENDSEKVDRLME LHFKYLGAMQVADKKIEGEK HDMVRRGEIIDNDTEEEFYL RRLDAGLFVLQHICYIMAE
Tissue Specificity Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid.
Function Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated somatic hypermutation (SHM) and class-switch recombination (CSR), 2 processes resulting in the production of high-affinity, mutated isotype-switched antibodies.
Protein Name Beta-Catenin-Like Protein 1
Nuclear-Associated Protein
Nap
Testis Development Protein Nyd-Sp19
Database Links Reactome: R-HSA-72163
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Beta-Catenin-Like Protein 1 antibody
Anti-Nuclear-Associated Protein antibody
Anti-Nap antibody
Anti-Testis Development Protein Nyd-Sp19 antibody
Anti-CTNNBL1 antibody
Anti-C20orf33 antibody
Anti-PP8304 antibody

Information sourced from Uniprot.org

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