• Western blot analysis of extracts of various cell lines, using CSK rabbit polyclonal antibody (STJ11102784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CSK antibody (STJ11102784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using CSK antibody (STJ11102784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CSK rabbit polyclonal antibody (STJ11102784) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CSK antibody (188-450) (STJ11102784)

SKU:
STJ11102784

€140.63 - €653.13
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSK (188-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSK
Gene ID: 1445
Uniprot ID: CSK_HUMAN
Immunogen Region: 188-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 188-450 of human CSK (NP_004374.1).
Immunogen Sequence: WALNMKELKLLQTIGKGEFG DVMLGDYRGNKVAVKCIKND ATAQAFLAEASVMTQLRHSN LVQLLGVIVEEKGGLYIVTE YMAKGSLVDYLRSRGRSVLG GDCLLKFSLDVCEAMEYLEG NNFVHRDLAARNVLVSEDNV AKVSDFGLTKEASSTQDTGK LPVKWTAPEALREKKFSTKS DVWSFGILLWEIYSFGRVPY PRIPLKDVVPRVEKGYKMDA PDGCPPAVYEVMKNCWHLD
Tissue Specificity Expressed in lung and macrophages.
Post Translational Modifications Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.
Function Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Protein Name Tyrosine-Protein Kinase Csk
C-Src Kinase
Protein-Tyrosine Kinase Cyl
Database Links Reactome: R-HSA-180292
Reactome: R-HSA-202427
Reactome: R-HSA-354192
Reactome: R-HSA-389948
Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9013407
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9706369
Cellular Localisation Cytoplasm
Cell Membrane
Mainly Cytoplasmic
Also Present In Lipid Rafts
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Csk antibody
Anti-C-Src Kinase antibody
Anti-Protein-Tyrosine Kinase Cyl antibody
Anti-CSK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance