Anti-CPT1A antibody (Internal) (STJ70998)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CPT1A (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80-85kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2kDa according to NP_001027017.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at ro |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CPT1A |
| Gene ID | 1374 |
| Uniprot ID | CPT1A_HUMAN |
| Accession Number | NP_001867.2; NP_001027017.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DPAQTVEQRLKLFK |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B. |
Additional Info
| Function | Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity. Plays an important role in hepatic triglyceride metabolism. Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression. Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation. Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways. |
| Protein Name | Carnitine O-Palmitoyltransferase 1 - Liver IsoformCpt1-LCarnitine O-Palmitoyltransferase I - Liver IsoformCpt ICpti-LCarnitine Palmitoyltransferase 1aSuccinyltransferase Cpt1a |
| Database Links | Reactome: R-HSA-1368082Reactome: R-HSA-1989781Reactome: R-HSA-200425Reactome: R-HSA-5362517 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Carnitine O-Palmitoyltransferase 1 - Liver Isoform antibodyAnti-Cpt1-L antibodyAnti-Carnitine O-Palmitoyltransferase I - Liver Isoform antibodyAnti-Cpt I antibodyAnti-Cpti-L antibodyAnti-Carnitine Palmitoyltransferase 1a antibodyAnti-Succinyltransferase Cpt1a antibodyAnti-CPT1A antibodyAnti-CPT1 antibody |
Information sourced from Uniprot.org