• STJ70636, (2ug/ml) staining of Human Liver (A) and (1ug/ml) Ovary (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70636 (0. 5ug/ml) staining of MCF7 (A) , (1ug/ml) U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70636 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-COMT antibody (N-Term) (STJ70636)

SKU:
STJ70636-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/FC
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-COMT (N-Term) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: COMT
Gene ID: 1312
Uniprot ID: COMT_HUMAN
Immunogen Region: N-Term
Accession Number: NP_000745.1; NP_001128633.1; NP_001128634.1; NP_009294.1
Specificity: This antibody is expected to recognise both reported isoforms S-COMT NP_009294.1 and MB-COMT NP_000745.1; NP_001128633.1; NP_001128634..
Immunogen Sequence: GDTKEQRILNHVLQC
Post Translational Modifications The N-terminus is blocked.
Function Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Protein Name Catechol O-Methyltransferase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-379397
Reactome: R-HSA-379398
Reactome: R-HSA-9679191
Cellular Localisation Isoform Soluble: Cytoplasm
Isoform Membrane-Bound: Cell Membrane
Single-Pass Type Ii Membrane Protein
Extracellular Side
Alternative Antibody Names Anti-Catechol O-Methyltransferase antibody
Anti-COMT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance