| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Complement C3 (1364-1663) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4784RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
C3 |
| Gene ID: |
12266 |
| Uniprot ID: |
CO3_MOUSE |
| Immunogen Region: |
1364-1663 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of mouse Complement C3 (NP_033908.2). |
| Immunogen Sequence: |
RVSIRPAPETAKKPEEAKNT MFLEICTKYLGDVDATMSIL DISMMTGFAPDTKDLELLAS GVDRYISKYEMNKAFSNKNT LIIYLEKISHTEEDCLTFKV HQYFNVGLIQPGSVKVYSYY NLEESCTRFYHPEKDDGMLS KLCHSEMCRCAEENCFMQQS QEKINLNVRLDKACEPGVDY VYKTELTNIELLDDFDEYTM TIQQVIKSGSDEVQAGQQRK FISHIKCRNALKLQKGKKY |
| Post Translational Modifications | C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons. Phosphorylated by FAM20C in the extracellular medium. |
| Function | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. |
| Protein Name | Complement C3Hse-Msf Cleaved Into - Complement C3 Beta Chain - C3-Beta-CC3bc - Complement C3 Alpha Chain - C3a Anaphylatoxin - Acylation Stimulating ProteinAspC3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 |
| Database Links | Reactome: R-MMU-173736Reactome: -MMU-174577Reactome: -MMU-198933Reactome: -MMU-375276Reactome: -MMU-381426Reactome: -MMU-418594Reactome: -MMU-6798695Reactome: -MMU-8957275Reactome: -MMU-977606 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Complement C3 antibodyAnti-Hse-Msf Cleaved Into - Complement C3 Beta Chain - C3-Beta-C antibodyAnti-C3bc - Complement C3 Alpha Chain - C3a Anaphylatoxin - Acylation Stimulating Protein antibodyAnti-Asp antibodyAnti-C3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 antibodyAnti-C3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
