• Western blot analysis of extracts of various cell lines, using COL4A5 antibody (STJ111851) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.

Anti-COL4A5 antibody (STJ111851)

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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against COL4A5.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: CO4A5_HUMAN
Gene ID: 1287
Gene Symbol: COL4A5
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1).
Background This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.
Tissue Specificity Isoform 2 is found in kidney.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Database Links Reactome: R-HSA-1442490
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Protein Name Collagen Alpha-5(Iv Chain
Alternative Names Anti-Collagen Alpha-5(Iv Chain antibody
Anti-COL4A5 antibody

Information sourced from uniprot.org

6 months standard