• Western blot analysis of extracts of various cell lines, using COL4A5 antibody (STJ111851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of A-431 cells using COL4A5 rabbit polyclonal antibody (STJ111851) at dilution of 1:1000 (40x lens). Blue: DAPI for nuclear staining.

Anti-COL4A5 antibody (1462-1691) (STJ111851)

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STJ111851

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL4A5 (1462-1691) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL4A5
Gene ID: 1287
Uniprot ID: CO4A5_HUMAN
Immunogen Region: 1462-1691
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1).
Immunogen Sequence: SSVAHGFLITRHSQTTDAPQ CPQGTLQVYEGFSLLYVQGN KRAHGQDLGTAGSCLRRFST MPFMFCNINNVCNFASRNDY SYWLSTPEPMPMSMQPLKGQ SIQPFISRCAVCEAPAVVIA VHSQTIQIPHCPQGWDSLWI GYSFMMHTSAGAEGSGQALA SPGSCLEEFRSAPFIECHGR GTCNYYANSYSFWLATVDVS DMFSKPQSETLKAGDLRTRI SRCQVCMKRT
Tissue Specificity Isoform 2 is found in kidney.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Protein Name Collagen Alpha-5(Iv Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Reactome: R-HSA-9010553
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Collagen Alpha-5(Iv Chain antibody
Anti-COL4A5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance