• STJ70757 (0. 003µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70757 (0. 001µg/ml) staining of Rat Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • HEK293 overexpressing Human Muscle Cofilin 2 (RC222215) and probed with STJ70757 (mock transfection in first lane) , tested by Origene.

Anti-Cofilin 2 antibody (C-Term) (STJ70757)

SKU:
STJ70757-100

$421.88
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Cofilin 2 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.0
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CFL2
Gene ID: 1073
Uniprot ID: COF2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_068733.1; NP_619579.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Immunogen Sequence: DIKDRSTLGEK
Post Translational Modifications The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Function Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.
Protein Name Cofilin-2
Cofilin - Muscle Isoform
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Colocalizes With Cspr3 In The Z Line Of Sarcomeres
Alternative Antibody Names Anti-Cofilin-2 antibody
Anti-Cofilin - Muscle Isoform antibody
Anti-CFL2 antibody

Information sourced from Uniprot.org

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