Anti-Cofilin 2 antibody (C-Term) (STJ70757)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70757-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Cofilin 2 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
ApplicationsPep-ELISA/WB/IHC
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Pig
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.0
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCFL2
Gene ID1073
Uniprot IDCOF2_HUMAN
Accession NumberNP_068733.1; NP_619579.1
Immunogen RegionC-Term
Immunogen SequenceDIKDRSTLGEK
SpecificityThis antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)

Additional Info

Post Translational Modifications The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Function Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.
Protein Name Cofilin-2
Cofilin - Muscle Isoform
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Colocalizes With Cspr3 In The Z Line Of Sarcomeres
Alternative Antibody Names Anti-Cofilin-2 antibody
Anti-Cofilin - Muscle Isoform antibody
Anti-CFL2 antibody

Information sourced from Uniprot.org

Citations

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