| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500 IHC-P:1:50-1:200 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CLIC4 |
| Gene ID: | 25932 |
| Uniprot ID: | CLIC4_HUMAN |
| Immunogen Region: | 1-253 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human CLIC4 (NP_039234.1). |
| Immunogen Sequence: | MALSMPLNGLKEEDKEPLIE LFVKAGSDGESIGNCPFSQR LFMILWLKGVVFSVTTVDLK RKPADLQNLAPGTHPPFITF NSEVKTDVNKIEEFLEEVLC PPKYLKLSPKHPESNTAGMD IFAKFSAYIKNSRPEANEAL ERGLLKTLQKLDEYLNSPLP DEIDENSMEDIKFSTRKFLD GNEMTLADCNLLPKLHIVKV VAKKYRNFDIPKEMTGIWRY LTNAYSRDEFTNTCPSDKE |
| Tissue Specificity | Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle. |
| Function | In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3. |
| Protein Name | Chloride Intracellular Channel Protein 4 Glutaredoxin-Like Oxidoreductase Clic4 Intracellular Chloride Ion Channel Protein P64h1 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Cytoplasmic Vesicle Membrane Single-Pass Membrane Protein Nucleus Cell Membrane Mitochondrion Cell Junction Endoplasmic Reticulum Membrane Colocalized With Akap9 At The Centrosome And Midbody Exists Both As Soluble Cytoplasmic Protein And As Membrane Protein With Probably A Single Transmembrane Domain Present In An Intracellular Vesicular Compartment That Likely Represent Trans-Golgi Network Vesicles Might Not Be Present In The Nucleus Of Cardiac Cells |
| Alternative Antibody Names | Anti-Chloride Intracellular Channel Protein 4 antibody Anti-Glutaredoxin-Like Oxidoreductase Clic4 antibody Anti-Intracellular Chloride Ion Channel Protein P64h1 antibody Anti-CLIC4 antibody |
Information sourced from Uniprot.org