• Western blot analysis of extracts of various cell lines, using CLEC1B antibody (STJ112012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using CLEC1B Rabbit polyclonal antibody (STJ112012) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CLEC1B antibody (22-196) (STJ112012)

SKU:
STJ112012

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLEC1B (22-196) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLEC1B
Gene ID: 51266
Uniprot ID: CLC1B_HUMAN
Immunogen Region: 22-196
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-196 of human CLEC1B (NP_001092901.1).
Immunogen Sequence: AVMQRNYLQGENENRTGTLQ QLAKRFCQYVVKQSELKGTF KGHKCSPCDTNWRYYGDSCY GFFRHNLTWEESKQYCTDMN ATLLKIDNRNIVEYIKARTH LIRWVGLSRQKSNEVWKWED GSVISENMFEFLEDGKGNMN CAYFHNGKMHPTFCENKHYL MCERKAGMTKVDQLP
Tissue Specificity Expressed preferentially in the liver. Also expressed in immune cells of myeloid origin and on the surface of platelets.
Post Translational Modifications Glycosylated. Phosphorylated on tyrosine residue in response to rhodocytin binding.
Function C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN. After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2. (Microbial infection) Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (SYK) and initiation of downstream tyrosine phosphorylation events and activation of PLCG2. (Microbial infection) Acts as an attachment factor for Human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.
Protein Name C-Type Lectin Domain Family 1 Member B
C-Type Lectin-Like Receptor 2
Clec-2
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-9707616
Cellular Localisation Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-C-Type Lectin Domain Family 1 Member B antibody
Anti-C-Type Lectin-Like Receptor 2 antibody
Anti-Clec-2 antibody
Anti-CLEC1B antibody
Anti-CLEC2 antibody
Anti-UNQ721 antibody
Anti-PRO1384 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance