• Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using Notch 1 (Cleaved-Val1754) Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1：500
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Notch 1 (Cleaved-Val1754) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1：500
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, Cleaved-Notch 1 (V1754) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, Cleaved-Notch 1 (V1754) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, Cleaved-Notch 1 (V1754) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Cleaved-Notch 1 (V1754) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Cleaved-NOTCH1-V1754 antibody (1735-1784 aa) (STJ90066)

SKU:
STJ90066

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Neurogenic locus notch homolog protein 1-V1754 (1735-1784 aa) is suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
IF 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOTCH1
Gene ID: 4851
Uniprot ID: NOTC1_HUMAN
Immunogen Region: 1735-1784 aa
Specificity: Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Notch 1 at the amino acid range 1735-1784
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by one of the catalytic subunits of gamma-secretase (PSEN1 or PSEN2), to release a Notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains. O-glucosylated at Ser-435 by KDELC1 and KDELC2. Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4. O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). MFNG-, RFNG- and LFNG-mediated modification of O-fucose residues at specific EGF-like domains results in inhibition of its activation by JAG1 and enhancement of its activation by DLL1 via an increased binding to DLL1. Ubiquitinated. Undergoes 'Lys-29'-linked polyubiquitination by ITCH.promotes the lysosomal degradation of non-activated internalized NOTCH1. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN. Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD.
Function Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis.negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Protein Name Neurogenic Locus Notch Homolog Protein 1
Notch 1
Hn1
Translocation-Associated Notch Protein Tan-1 Cleaved Into - Notch 1 Extracellular Truncation
Next - Notch 1 Intracellular Domain
Nicd
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2644607
Reactome: R-HSA-2660826
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-8941856
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9793380
Reactome: R-HSA-9818749
Reactome: R-HSA-9824272
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 1 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Nuclear Location May Require Megf10
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 1 antibody
Anti-Notch 1 antibody
Anti-Hn1 antibody
Anti-Translocation-Associated Notch Protein Tan-1 Cleaved Into - Notch 1 Extracellular Truncation antibody
Anti-Next - Notch 1 Intracellular Domain antibody
Anti-Nicd antibody
Anti-NOTCH1 antibody
Anti-TAN1 antibody

Information sourced from Uniprot.org

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