| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cleaved-BH3-interacting domain death agonist-Ser100 (99-100) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BID |
| Gene ID: |
637 |
| Uniprot ID: |
BID_HUMAN |
| Immunogen Region: |
99-100 |
| Specificity: |
This antibody detects endogenous levels of Human, Mouse BID (p11, Cleaved-Ser100, protein was cleaved amino acid sequence between 99-100) |
| Immunogen: |
Synthesized peptide derived from human BID (p11, Cleaved-Ser100) |
| Tissue Specificity | Isoform 2: Expressed in spleen, pancreas and placenta (at protein level). Isoform 3: Expressed in lung, pancreas and spleen (at protein level). Isoform 4: Expressed in lung and pancreas (at protein level). |
| Post Translational Modifications | BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation. |
| Function | Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis. |
| Protein Name | Bh3-Interacting Domain Death AgonistP22 BidBid Cleaved Into - Bh3-Interacting Domain Death Agonist P15P15 Bid - Bh3-Interacting Domain Death Agonist P13P13 Bid - Bh3-Interacting Domain Death Agonist P11P11 Bid |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111452Reactome: R-HSA-111453Reactome: R-HSA-114294Reactome: R-HSA-6803204Reactome: R-HSA-75108 |
| Cellular Localisation | CytoplasmMitochondrion MembraneMitochondrion Outer MembraneWhen UncleavedIt Is Predominantly CytoplasmicBh3-Interacting Domain Death Agonist P15: Mitochondrion MembraneTranslocates To Mitochondria As An Integral Membrane ProteinBh3-Interacting Domain Death Agonist P13: Mitochondrion MembraneAssociated With The Mitochondrial MembraneIsoform 1: CytoplasmIsoform 3: CytoplasmIsoform 2: Mitochondrion MembraneA Significant Proportion Of Isoform 2 Localizes To MitochondriaIt May Be Cleaved Constitutively |
| Alternative Antibody Names | Anti-Bh3-Interacting Domain Death Agonist antibodyAnti-P22 Bid antibodyAnti-Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 antibodyAnti-P15 Bid - Bh3-Interacting Domain Death Agonist P13 antibodyAnti-P13 Bid - Bh3-Interacting Domain Death Agonist P11 antibodyAnti-P11 Bid antibodyAnti-BID antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
