Anti-Cleaved-BID-Ser100 antibody (99-100) (STJA0005820)

SKU:
STJA0005820

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-BH3-interacting domain death agonist-Ser100 (99-100) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BID
Gene ID: 637
Uniprot ID: BID_HUMAN
Immunogen Region: 99-100
Specificity: This antibody detects endogenous levels of Human, Mouse BID (p11, Cleaved-Ser100, protein was cleaved amino acid sequence between 99-100)
Immunogen: Synthesized peptide derived from human BID (p11, Cleaved-Ser100)
Tissue Specificity Isoform 2: Expressed in spleen, pancreas and placenta (at protein level). Isoform 3: Expressed in lung, pancreas and spleen (at protein level). Isoform 4: Expressed in lung and pancreas (at protein level).
Post Translational Modifications BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation.
Function Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis.
Protein Name Bh3-Interacting Domain Death Agonist
P22 Bid
Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15
P15 Bid - Bh3-Interacting Domain Death Agonist P13
P13 Bid - Bh3-Interacting Domain Death Agonist P11
P11 Bid
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111452
Reactome: R-HSA-111453
Reactome: R-HSA-114294
Reactome: R-HSA-6803204
Reactome: R-HSA-75108
Cellular Localisation Cytoplasm
Mitochondrion Membrane
Mitochondrion Outer Membrane
When Uncleaved
It Is Predominantly Cytoplasmic
Bh3-Interacting Domain Death Agonist P15: Mitochondrion Membrane
Translocates To Mitochondria As An Integral Membrane Protein
Bh3-Interacting Domain Death Agonist P13: Mitochondrion Membrane
Associated With The Mitochondrial Membrane
Isoform 1: Cytoplasm
Isoform 3: Cytoplasm
Isoform 2: Mitochondrion Membrane
A Significant Proportion Of Isoform 2 Localizes To Mitochondria
It May Be Cleaved Constitutively
Alternative Antibody Names Anti-Bh3-Interacting Domain Death Agonist antibody
Anti-P22 Bid antibody
Anti-Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 antibody
Anti-P15 Bid - Bh3-Interacting Domain Death Agonist P13 antibody
Anti-P13 Bid - Bh3-Interacting Domain Death Agonist P11 antibody
Anti-P11 Bid antibody
Anti-BID antibody

Information sourced from Uniprot.org

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