Anti-CKB antibody (182-381) (STJ114505)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CKB (182-381) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CKB |
| Gene ID | 1152 |
| Uniprot ID | KCRB_HUMAN |
| Immunogen Region | 182-381 |
| Immunogen Sequence | AEQQQLIDDHFLFDKPVSPL LLASGMARDWPDARGIWHND NKTFLVWVNEEDHLRVISMQ KGGNMKEVFTRFCTGLTQIE TLFKSKDYEFMWNPHLGYIL TCPSNLGTGLRAGVHIKLPN LGKHEKFSEVLKRLRLQKRG TGGVDTAAVGGVFDVSNADR LGFSEVELVQMVVDGVKLLI EMEQRLEQGQAIDDLMPAQK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human Creatine kinase B type (Creatine kinase B type (CKB) ) (NP_001814.2). |
Additional Info
| Post Translational Modifications | Ubiquitinated by the ECS(ASB9) complex, leading to its degradation by the proteasome. |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Creatine Kinase B-TypeBrain Creatine KinaseB-CkCreatine Kinase B ChainCreatine Phosphokinase B-TypeCpk-B |
| Database Links | Reactome: R-HSA-71288Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmCytosolMitochondrionCell MembraneLocalizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region |
| Alternative Antibody Names | Anti-Creatine Kinase B-Type antibodyAnti-Brain Creatine Kinase antibodyAnti-B-Ck antibodyAnti-Creatine Kinase B Chain antibodyAnti-Creatine Phosphokinase B-Type antibodyAnti-Cpk-B antibodyAnti-CKB antibodyAnti-CKBB antibody |
Information sourced from Uniprot.org