• Immunocytochemistry staining of HeLa cells fixed with-20℃ Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
  • Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.
  • Western blot detection of CHD3 (C-terminus) in NTERA2, 3T3, C6, K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.

Anti-CHD3 antibody (C-Term) [7F6-G2-E5] (STJ99071)

SKU:
STJ99071

$187.65 - $453.60
Current Stock:
Host: Mouse
Applications: WB/ICC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Chromodomain-helicase-DNA-binding protein 3 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 7F6-G2-E5
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:400
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD3
Gene ID: 1107
Uniprot ID: CHD3_HUMAN
Immunogen Region: C-Term
Specificity: This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
Immunogen: Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
Post Translational Modifications Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression.
Function ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 3
Chd-3
Atp-Dependent Helicase Chd3
Mi-2 Autoantigen 240 Kda Protein
Mi2-Alpha
Zinc Finger Helicase
Hzfh
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-4551638
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Pml Body
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Associates With Centrosomes In Interphase And Mitosis
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 3 antibody
Anti-Chd-3 antibody
Anti-Atp-Dependent Helicase Chd3 antibody
Anti-Mi-2 Autoantigen 240 Kda Protein antibody
Anti-Mi2-Alpha antibody
Anti-Zinc Finger Helicase antibody
Anti-Hzfh antibody
Anti-CHD3 antibody

Information sourced from Uniprot.org

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