| Host: |
Mouse |
| Applications: |
WB/ICC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Chromodomain-helicase-DNA-binding protein 3 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
7F6-G2-E5 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000ICC 1:400 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHD3 |
| Gene ID: |
1107 |
| Uniprot ID: |
CHD3_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins. |
| Immunogen: |
Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. |
| Post Translational Modifications | Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression. |
| Function | ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. |
| Protein Name | Chromodomain-Helicase-Dna-Binding Protein 3Chd-3Atp-Dependent Helicase Chd3Mi-2 Autoantigen 240 Kda ProteinMi2-AlphaZinc Finger HelicaseHzfh |
| Database Links | Reactome: R-HSA-3214815Reactome: R-HSA-427389Reactome: R-HSA-4551638Reactome: R-HSA-6804758Reactome: R-HSA-73762Reactome: R-HSA-8943724Reactome: R-HSA-9679191 |
| Cellular Localisation | NucleusPml BodyCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With Centrosomes In Interphase And MitosisLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Chromodomain-Helicase-Dna-Binding Protein 3 antibodyAnti-Chd-3 antibodyAnti-Atp-Dependent Helicase Chd3 antibodyAnti-Mi-2 Autoantigen 240 Kda Protein antibodyAnti-Mi2-Alpha antibodyAnti-Zinc Finger Helicase antibodyAnti-Hzfh antibodyAnti-CHD3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Standard Curve for Human Cystatin C: Capture Antibody Mouse mAb [6F12-C7-D8] to Human Cystatin C at 4 Mu g/ml and Detector Antibody Mouse mAb [7F6-A5-F3]to Human Cystatin C at 0.5 Mu g/ml.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/121336/232247/STJ99196_1_ELISA__42541.1602855627.jpg?c=1 "Standard Curve for Human Cystatin C: Capture Antibody Mouse mAb [6F12-C7-D8] to Human Cystatin C at 4 Mu g/ml and Detector Antibody Mouse mAb [7F6-A5-F3]to Human Cystatin C at 0.5 Mu g/ml.")
