• Flow cytometric analysis of K562 cells using Mi2-Alpha monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded colon cancer tissues with DAB staining using Mi2-Alpha monoclonal antibody.
  • Immunofluorescence analysis of Hela cells using Mi2-Alpha monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using Mi2-Alpha monoclonal antibody against HeLa (1) , K562 (2) , Jurkat (3) , NTERA-2 (4) , HEK293 (5) , Raji (6) cell lysate and mouse brain (7) tissue lysate.

Anti-CHD3 antibody [2G4] (STJ98244)

SKU:
STJ98244

$187.65 - $453.60
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Chromodomain-helicase-DNA-binding protein 3 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 2G4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD3
Gene ID: 1107
Uniprot ID: CHD3_HUMAN
Specificity: Mi2-Alpha Monoclonal Antibody detects endogenous levels of Mi2-Alpha protein.
Immunogen: Purified recombinant fragment of human Mi2-Alpha expressed in E. Coli.
Post Translational Modifications Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression.
Function ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 3
Chd-3
Atp-Dependent Helicase Chd3
Mi-2 Autoantigen 240 Kda Protein
Mi2-Alpha
Zinc Finger Helicase
Hzfh
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-4551638
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Pml Body
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Associates With Centrosomes In Interphase And Mitosis
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 3 antibody
Anti-Chd-3 antibody
Anti-Atp-Dependent Helicase Chd3 antibody
Anti-Mi-2 Autoantigen 240 Kda Protein antibody
Anti-Mi2-Alpha antibody
Anti-Zinc Finger Helicase antibody
Anti-Hzfh antibody
Anti-CHD3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance