Anti-CHAF1B antibody (270-559) (STJ28015)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CHAF1B (270-559) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHAF1B |
| Gene ID | 8208 |
| Uniprot ID | CAF1B_HUMAN |
| Immunogen Region | 270-559 |
| Immunogen Sequence | SRKNLKRPIAHLPCPGKATL AVRCCPVYFELRPVVETGVE LMSLPYRLVFAVASEDSVLL YDTQQSFPFGYVSNIHYHTL SDISWSSDGAFLAISSTDGY CSFVTFEKDELGIPLKEKPV LNMRTPDTAKKTKSQTHRGS SPGPRPVEGTPASRTQDPSS PGTTPPQARQAPAPTVIRDP PSITPAVKSPLPGPSEEKTL QPSSQNTKAHPSRRVTLNTL QAWSKTTPRRINLTPLKTD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human p60 CAF1 (NP_005432.1). |
Additional Info
| Post Translational Modifications | Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. |
| Function | Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA.histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. |
| Protein Name | Chromatin Assembly Factor 1 Subunit BCaf-1 Subunit BChromatin Assembly Factor I P60 SubunitCaf-I 60 Kda SubunitCaf-I P60M-Phase Phosphoprotein 7 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmDna Replication FociCytoplasmic In M Phase |
| Alternative Antibody Names | Anti-Chromatin Assembly Factor 1 Subunit B antibodyAnti-Caf-1 Subunit B antibodyAnti-Chromatin Assembly Factor I P60 Subunit antibodyAnti-Caf-I 60 Kda Subunit antibodyAnti-Caf-I P60 antibodyAnti-M-Phase Phosphoprotein 7 antibodyAnti-CHAF1B antibodyAnti-CAF1A antibodyAnti-CAF1P60 antibodyAnti-MPHOSPH7 antibodyAnti-MPP7 antibody |
Information sourced from Uniprot.org