| Short Description : | Rabbit polyclonal CHAC1 (1-222) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHAC1 |
| Gene ID: | 79094 |
| Uniprot ID: | CHAC1_HUMAN |
| Immunogen Region: | 1-222 |
| Immunogen Sequence: | MKQESAAPNTPPTSQSPTPS AQFPRNDGDPQALWIFGYGS LVWRPDFAYSDSRVGFVRGY SRRFWQGDTFHRGSDKMPGR VVTLLEDHEGCTWGVAYQVQ GEQVSKALKYLNVREAVLGG YDTKEVTFYPQDAPDQPLKA LAYVATPQNPGYLGPAPEEA IATQILACRGFSGHNLEYLL RLADFMQLCGPQAQDEHLAA IVDAVGTMLPCFCPTEQALA LV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human CHAC1 (NP_077016.3). |
| Function | Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. |
| Protein Name | Glutathione-Specific Gamma-Glutamylcyclotransferase 1Gamma-Gcg 1Blocks Notch ProteinBotchCation Transport Regulator-Like Protein 1 |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-9648895 |
| Cellular Localisation | CytoplasmCytosolGolgi ApparatusTrans-Golgi Network |
| Alternative Antibody Names | Anti-Glutathione-Specific Gamma-Glutamylcyclotransferase 1 antibodyAnti-Gamma-Gcg 1 antibodyAnti-Blocks Notch Protein antibodyAnti-Botch antibodyAnti-Cation Transport Regulator-Like Protein 1 antibodyAnti-CHAC1 antibodyAnti-BOTCH antibody |
Information sourced from Uniprot.org
