• Immunofluorescence analysis of Raw264. 7 cells using Complement factor B (CFB) Rabbit polyclonal antibody (STJ23103) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Complement factor B (CFB) in paraffin-embedded mouse lung using Complement factor B (CFB) Rabbit polyclonal antibody (STJ23103) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Complement factor B (CFB) in paraffin-embedded mouse heart using Complement factor B (CFB) Rabbit polyclonal antibody (STJ23103) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Complement factor B (CFB) in paraffin-embedded human mammary cancer using Complement factor B (CFB) Rabbit polyclonal antibody (STJ23103) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Complement factor B (CFB) Rabbit polyclonal antibody (STJ23103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CFB antibody (26-259) (STJ23103)

SKU:
STJ23103

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Complement Factor B (26-259) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFB
Gene ID: 629
Uniprot ID: CFAB_HUMAN
Immunogen Region: 26-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-259 of Complement factor B (CFB) (NP_001701.2).
Immunogen Sequence: TPWSLARPQGSCSLEGVEIK GGSFRLLQEGQALEYVCPSG FYPYPVQTRTCRSTGSWSTL KTQDQKTVRKAECRAIHCPR PHDFENGEYWPRSPYYNVSD EISFHCYDGYTLRGSANRTC QVNGRWSGQTAICDNGAGYC SNPGIPIGTRKVGSQYRLED SVTYHCSRGLTLRGSQRRTC QEGGSWSGTEPSCQDSFMYD TPQEVAEAFLSSLTETIEGV DAEDGHGPGEQQKR
Function Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Protein Name Complement Factor B
C3/C5 Convertase
Glycine-Rich Beta Glycoprotein
Gbg
Pbf2
Properdin Factor B Cleaved Into - Complement Factor B Ba Fragment - Complement Factor B Bb Fragment
Database Links Reactome: R-HSA-173736
Reactome: R-HSA-174577
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement Factor B antibody
Anti-C3/C5 Convertase antibody
Anti-Glycine-Rich Beta Glycoprotein antibody
Anti-Gbg antibody
Anti-Pbf2 antibody
Anti-Properdin Factor B Cleaved Into - Complement Factor B Ba Fragment - Complement Factor B Bb Fragment antibody
Anti-CFB antibody
Anti-BF antibody
Anti-BFD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance