• Western blot analysis of extracts of various cell lines, using CES1 rabbit monoclonal antibody (STJ11101340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of HepG2 cells, using CES1 rabbit monoclonal antibody (STJ11101340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa using CES1 rabbit monoclonal antibody (STJ11101340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver using CES1 rabbit monoclonal antibody (STJ11101340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver using CES1 rabbit monoclonal antibody (STJ11101340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CES1 antibody (1-100) [S0MR] (STJ11101340)

SKU:
STJ11101340

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CES1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CES1
Gene ID: 1066
Uniprot ID: EST1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CES1 (P23141).
Immunogen Sequence: MWLRAFILATLSASAAWGHP SSPPVVDTVHGKVLGKFVSL EGFAQPVAIFLGIPFAKPPL GPLRFTPPQPAEPWSFVKNA TSYPPMCTQDPKAGQLLSEL
Tissue Specificity Expressed predominantly in liver with lower levels in heart and lung. Expressed in macrophages.
Post Translational Modifications Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins. Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process. First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile.
Protein Name Liver Carboxylesterase 1
Acyl-Coenzyme A -Cholesterol Acyltransferase
Acat
Brain Carboxylesterase Hbr1
Carboxylesterase 1
Ce-1
Hce-1
Cholesteryl Ester Hydrolase
Ceh
Cocaine Carboxylesterase
Egasyn
Hmse
Methylumbelliferyl-Acetate Deacetylase 1
Monocyte/Macrophage Serine Esterase
Retinyl Ester Hydrolase
Reh
Serine Esterase 1
Triacylglycerol Hydrolase
Tgh
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-211945
Reactome: R-HSA-5578768
Reactome: R-HSA-9749641
Cellular Localisation Endoplasmic Reticulum Lumen
Cytoplasm
Lipid Droplet
Moves From Cytoplasm To Lipid Droplets Upon Lipid Loading
Associates With Lipid Droplets Independently Of Triglycerides (Tg) Content Of The Droplets And Hydrolyzes Cholesteryl Esters More Efficiently From Mixed Droplets
Alternative Antibody Names Anti-Liver Carboxylesterase 1 antibody
Anti-Acyl-Coenzyme A -Cholesterol Acyltransferase antibody
Anti-Acat antibody
Anti-Brain Carboxylesterase Hbr1 antibody
Anti-Carboxylesterase 1 antibody
Anti-Ce-1 antibody
Anti-Hce-1 antibody
Anti-Cholesteryl Ester Hydrolase antibody
Anti-Ceh antibody
Anti-Cocaine Carboxylesterase antibody
Anti-Egasyn antibody
Anti-Hmse antibody
Anti-Methylumbelliferyl-Acetate Deacetylase 1 antibody
Anti-Monocyte/Macrophage Serine Esterase antibody
Anti-Retinyl Ester Hydrolase antibody
Anti-Reh antibody
Anti-Serine Esterase 1 antibody
Anti-Triacylglycerol Hydrolase antibody
Anti-Tgh antibody
Anti-CES1 antibody
Anti-CES2 antibody
Anti-SES1 antibody

Information sourced from Uniprot.org

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