Anti-CEP68 antibody (1-350) (STJ117058)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CEP68 (1-350) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CEP68 |
| Gene ID | 23177 |
| Uniprot ID | CEP68_HUMAN |
| Immunogen Region | 1-350 |
| Immunogen Sequence | MALGEEKAEAEASEDTKAQS YGRGSCRERELDIPGPMSGE QPPRLEAEGGLISPVWGAEG IPAPTCWIGTDPGGPSRAHQ PQASDANREPVAERSEPALS GLPPATMGSGDLLLSGESQV EKTKLSSSEEFPQTLSLPRT TTICSGHDADTEDDPSLADL PQALDLSQQPHSSGLSCLSQ WKSVLSPGSAAQPSSCSISA SSTGSSLQGHQERAEPRGGS LAKVSSSLEPVVPQEPSSV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CEP68 (NP_001306030.1). |
Additional Info
| Post Translational Modifications | Phosphorylation by PLK1 is required for binding to BTRC in prometaphase. Phosphorylated directly or indirectly by NEK2. NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes. Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation. |
| Function | Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting. Required for localization of CDK5RAP2 to the centrosome during interphase. Contributes to CROCC/rootletin filament formation. |
| Protein Name | Centrosomal Protein Of 68 KdaCep68 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes To Thin Fibers Protruding Away From The Proximal Ends Of The Two CentriolesDissociates From Interphase Centrosomes At The Onset Of Mitosis |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 68 Kda antibodyAnti-Cep68 antibodyAnti-CEP68 antibodyAnti-KIAA0582 antibody |
Information sourced from Uniprot.org