| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CEP68 (1-350) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEP68 |
| Gene ID: |
23177 |
| Uniprot ID: |
CEP68_HUMAN |
| Immunogen Region: |
1-350 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CEP68 (NP_001306030.1). |
| Immunogen Sequence: |
MALGEEKAEAEASEDTKAQS YGRGSCRERELDIPGPMSGE QPPRLEAEGGLISPVWGAEG IPAPTCWIGTDPGGPSRAHQ PQASDANREPVAERSEPALS GLPPATMGSGDLLLSGESQV EKTKLSSSEEFPQTLSLPRT TTICSGHDADTEDDPSLADL PQALDLSQQPHSSGLSCLSQ WKSVLSPGSAAQPSSCSISA SSTGSSLQGHQERAEPRGGS LAKVSSSLEPVVPQEPSSV |
| Post Translational Modifications | Phosphorylation by PLK1 is required for binding to BTRC in prometaphase. Phosphorylated directly or indirectly by NEK2. NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes. Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation. |
| Function | Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting. Required for localization of CDK5RAP2 to the centrosome during interphase. Contributes to CROCC/rootletin filament formation. |
| Protein Name | Centrosomal Protein Of 68 KdaCep68 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes To Thin Fibers Protruding Away From The Proximal Ends Of The Two CentriolesDissociates From Interphase Centrosomes At The Onset Of Mitosis |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 68 Kda antibodyAnti-Cep68 antibodyAnti-CEP68 antibodyAnti-KIAA0582 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
