| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Centrosome-associated protein CEP250 is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC-P 1:50-300IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEP250 |
| Gene ID: |
11190 |
| Uniprot ID: |
CP250_HUMAN |
| Specificity: |
CP250 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from part of the human protein |
| Post Translational Modifications | Differentially phosphorylated during cell cycle. Phosphorylation may regulate association/dissociation from centrosome. During M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome. Dephosphorylated in vitro by the PP1 phosphatase. |
| Function | May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase. Recruits CCDC102B to the proximal ends of centrioles. |
| Protein Name | Centrosome-Associated Protein Cep250250 Kda Centrosomal ProteinCep250Centrosomal Nek2-Associated Protein 1C-Nap1Centrosomal Protein 2 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmPerinuclear RegionCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyCell ProjectionCiliumPhotoreceptor Outer SegmentPhotoreceptor Inner SegmentComponent Of The Core CentrosomeIn Interphase CellsIt Specifically Associates With The Proximal Ends Of Both Mother And Daughter CentriolesAssociates With The Centrosome In Interphase CellsIn Mitotic CellsIt Dissociates From The Mitotic Spindle PolesAt The End Of Cell DivisionIt Reaccumulates At Centrosomes |
| Alternative Antibody Names | Anti-Centrosome-Associated Protein Cep250 antibodyAnti-250 Kda Centrosomal Protein antibodyAnti-Cep250 antibodyAnti-Centrosomal Nek2-Associated Protein 1 antibodyAnti-C-Nap1 antibodyAnti-Centrosomal Protein 2 antibodyAnti-CEP250 antibodyAnti-CEP2 antibodyAnti-CNAP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
