• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CEP250 antibody (STJ190683)

SKU:
STJ190683

€60.00 - €315.00
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Centrosome-associated protein CEP250 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-300
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEP250
Gene ID: 11190
Uniprot ID: CP250_HUMAN
Specificity: CP250 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Post Translational Modifications Differentially phosphorylated during cell cycle. Phosphorylation may regulate association/dissociation from centrosome. During M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome. Dephosphorylated in vitro by the PP1 phosphatase.
Function May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase. Recruits CCDC102B to the proximal ends of centrioles.
Protein Name Centrosome-Associated Protein Cep250
250 Kda Centrosomal Protein
Cep250
Centrosomal Nek2-Associated Protein 1
C-Nap1
Centrosomal Protein 2
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Perinuclear Region
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Cell Projection
Cilium
Photoreceptor Outer Segment
Photoreceptor Inner Segment
Component Of The Core Centrosome
In Interphase Cells
It Specifically Associates With The Proximal Ends Of Both Mother And Daughter Centrioles
Associates With The Centrosome In Interphase Cells
In Mitotic Cells
It Dissociates From The Mitotic Spindle Poles
At The End Of Cell Division
It Reaccumulates At Centrosomes
Alternative Antibody Names Anti-Centrosome-Associated Protein Cep250 antibody
Anti-250 Kda Centrosomal Protein antibody
Anti-Cep250 antibody
Anti-Centrosomal Nek2-Associated Protein 1 antibody
Anti-C-Nap1 antibody
Anti-Centrosomal Protein 2 antibody
Anti-CEP250 antibody
Anti-CEP2 antibody
Anti-CNAP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance