• Immunofluorescence analysis of C6 cells using CENPC Rabbit monoclonal antibody (STJ11103543) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CENPC Rabbit monoclonal antibody (STJ11103543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CENPC antibody (380-520) [S3MR] (STJ11103543)

SKU:
STJ11103543

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CENPC (380-520) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPC
Gene ID: 1060
Uniprot ID: CENPC_HUMAN
Immunogen Region: 380-520
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-520 of human CENPC (Q03188).
Immunogen Sequence: VLDTSYALIGETVNNYRSTK YEMYSKNAEKPSRSKRTIKQ KQRRKFMAKPAEEQLDVGQS KDENIHTSHITQDEFQRNSD RNMEEHEEMGNDCVSKKQMP PVGSKKSSTRKDKEESKKKR FSSESKNKLVPEEVTSTVTK S
Function Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
Protein Name Centromere Protein C
Cenp-C
Centromere Autoantigen C
Centromere Protein C 1
Cenp-C 1
Interphase Centromere Complex Protein 7
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-606279
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes Exclusively In The Kinetochore Domain Of Centromeres
Alternative Antibody Names Anti-Centromere Protein C antibody
Anti-Cenp-C antibody
Anti-Centromere Autoantigen C antibody
Anti-Centromere Protein C 1 antibody
Anti-Cenp-C 1 antibody
Anti-Interphase Centromere Complex Protein 7 antibody
Anti-CENPC antibody
Anti-CENPC1 antibody
Anti-ICEN7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance