• Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK6 antibody (STJ11103846). Western blot was performed from the immunoprecipitate using CDK6 antibody (STJ11103846) at a dilution of 1:1000.
  • Immunohistochemistry analysis of CDK6 in paraffin-embedded human colon carcinoma using CDK6 Rabbit polyclonal antibody (STJ11103846) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using CDK6 Rabbit polyclonal antibody (STJ11103846) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CDK6 antibody (226-326) (STJ11103846)

SKU:
STJ11103846

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclin-Dependent Kinase 6 (226-326) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK6
Gene ID: 1021
Uniprot ID: CDK6_HUMAN
Immunogen Region: 226-326
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 226-326 of human CDK6 (NP_001250.1).
Immunogen Sequence: DQLGKILDVIGLPGEEDWPR DVALPRQAFHSKSAQPIEKF VTDIDELGKDLLLKCLTFNP AKRISAYSALSHPYFQDLER CKENLDSHLPPSQNTSELNT A
Tissue Specificity Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
Post Translational Modifications Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
Function Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation.promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation.prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.
Protein Name Cyclin-Dependent Kinase 6
Cell Division Protein Kinase 6
Serine/Threonine-Protein Kinase Plstire
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-69231
Reactome: R-HSA-8934593
Reactome: R-HSA-9630794
Reactome: R-HSA-9632700
Reactome: R-HSA-9661069
Reactome: R-HSA-9754119
Cellular Localisation Cytoplasm
Nucleus
Cell Projection
Ruffle
Cytoskeleton
Microtubule Organizing Center
Centrosome
Localized To The Ruffling Edge Of Spreading Fibroblasts
Kinase Activity Only In Nucleus
Localized To The Cytosol Of Neurons And Showed Prominent Staining Around Either Side Of The Nucleus
Present In The Cytosol And In The Nucleus In Interphase Cells And At The Centrosome During Mitosis From Prophase To Telophase
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 6 antibody
Anti-Cell Division Protein Kinase 6 antibody
Anti-Serine/Threonine-Protein Kinase Plstire antibody
Anti-CDK6 antibody
Anti-CDKN6 antibody

Information sourced from Uniprot.org

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